The following databases contain transcript sequences: Reference mRNA (refseq_mrna), Nucleotide collection (nr/nt), and the EST databases.Ĭlick the BLAST button to run the search and identify matching sequences. interest (+/- 2 kb) was retrieved and primers were designed using SnapGene. For a protein sequence, select the blastx translating service. FASP is formerly known as familial advanced sleep phase syndrome and currently referred to as familial advanced sleep phase disorder. In the 'Import' window that opens, set the name for the new Collection, and use the Browse button to specify where the new Collection will be saved, then. Alternatively, click File Open and select the file in the file picker. Drag and drop a multisequence file into the SnapGene Launch window. Use the NCBI BLAST service to perform a similarity search.įor a nucleotide sequence select the nucleotide blast service from the Basic BLAST section of the BLAST home page. PER2 is the first gene found to carry mutation that causes FASP. Import a Multisequence File as a New Collection. Include the organism in the search to find the most relevant results and filter for transcript sequences, for example: Cytochrome c AND bullfrog AND mrna. If there is no UniGene cluster for this gene and organism, perform a search in the Nucleotide database with the gene name, product name, or symbol.
The UniGene cluster has links to transcript sequences for the gene from the Nucleotide and EST databases If there is no gene record for the organism and gene of interest, perform a search in the UniGene database with the gene name, product name, or symbol.Ĭlick on the UniGene cluster of interest.
#Per2 snapgene software#
Use with SnapGene software or the free Viewer to visualize additional data and align other sequences. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Transcript sequences are not produced for prokaryotes. Use text editor or plasmid mapping software to view sequence. The NCBI Reference Sequences section of the record has links to NCBI curated records for transcripts (NM and XM prefix reference sequences) for the gene of interest for eukaryotic organisms. Northern blots analysis revealed that PER2 was expressed as a 7-kb mRNA in all tissues examined. 9 Predicted human PER2 protein created from the PER2 gene shares 44 identity with human PER1 and 77 identity with mouse Per2. If you know the gene symbol and species, enter them as follows: tpo AND humanĬlick on Reference Sequences in the Table of Contents at the upper right of the gene record. Gene edit The PER2 gene is located on the long (q) arm of chromosome 2 at position 37.3 and has 25 exons. Search the Gene database with the gene name, symbol.
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